Structural genomics and immunoinformatics analyses of non-structural protein 6 (NSP6) and its probable role in autophagy

Autophagy is a natural cellular mechanism in which components such as long-lived proteins and damaged organelles are degraded response to starvation by forming autophagosomes. Viruses activate the autophagy process, generates innate immune protection host against infection. While actual molecular of this contagious viral infection remains unknown, studies on some other betacoronavirus show that their cells inhibits resulting autophagosome accumulation inside cells. Non-structural protein 6 (NSP6) crucial blocking autophagosomes/autolysosome vesicle formation, more numerous smaller than autophagosomes formed upon starvation. Because its vital role autophagy, NSP6 can be used an effective drug target combat severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infections. Therefore, study aims detect mutations Indian isolates compared Wuhan-type isolates. The Indiantype SARS-CoV-2 contained 654 point mutations. Furthermore, secondary structure, energy change mutation, physicochemical properties, hydropathy index wild mutated were compared, clearly showing altered stability. An immunoinformatics approach was also attempted identify B-cell interferon (IFN)-inducing epitopes for using probable vaccine candidate. explored important essential assembly replicase proteins.